How to Inoculate Your Grain Spawn Bag

  • Prepare

    • Clean the injection port with an alcohol wipe.
  • Step 1 Inject

    Inject

    • Quickly insert the syringe into the self-healing injection port and
    • Inject 10cc of liquid culture or spores.

  • Store

    • Store the bag in a dark, warm area (70-75°F) for colonization.

  • Shake

    • When the mycelium has colonized 40-50% of the grain, gently massage the bag for 10 seconds, flip it, and repeat.
    • Finally, shake the bag to ensure even distribution of the mycelium.

  • Monitor

    • Check periodically for complete colonization. When the grain is fully white, it’s ready for use.

Shaking the bag helps the mycelium access uncolonized grain patches, boosting your yield. Avoid shaking too often to prevent disrupting the colonization process.

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