How to Use Mono Tub

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    PREPARE THE MONO TUB

    • Clean the tub: Wipe down the interior with 70% isopropyl alcohol or another sanitizing solution.
    • Attach Micropose filters.
    • Add a liner (optional): Line the bottom of the tub with a black trash bag or similar material to prevent side pinning.

  • ADD SUBSTRATE

    • Layer the substrate: Spread 2–3 inches of hydrated substrate evenly on the bottom of the tub.

  • ADD COLONIZED GRAIN SPAWN

    • Break up your colonized grain spawn into small pieces and mix it evenly with the substrate.
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    TOP LAYER

    • Add a thin layer of substrate (¼–½ inch) over the spawn to create an even surface. This optional step helps with even colonization.

  • INCUBATE FOR COLONIZATION

    • Place the lid on the tub and keep the filters in place for proper gas exchange (not full fresh air exchange).
    • Store the tub in a warm, dark location (70-78°F). Avoid direct sunlight or overly humid conditions.
    • Wait 10–14 days for the substrate to fully colonize. You'll know it's ready when the surface is covered with white mycelium.

  • FRUITING CONDITIONS

    • Once the substrate is fully colonized, introduce fruiting conditions by lightly misting the surface if it looks dry.
    • Fan the tub once or twice daily if additional fresh air exchange is needed.

HARVESTING

  • Harvest mushrooms when the veil under the cap begins to tear away from the stem.
  • Remove the spent substrate after the flushes are complete.
  • Wash and sanitize the tub before reusing it for your next grow.

Results may vary, largely dependent on having a clean liquid culture and a sterile process.

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